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1996. Ultrasensitive detection of phytoplasmas by nested-PCR assays utilizing two common primer pairs. 480 kb were achieved in symptomatic Spilanthes leaf samples from all of the three areas with the phytoplasma particular common 16S rRNA and secA gene specific primers respectively.
1992. Detection of DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Sequence comparison evaluation of 16S rRNA and secA genes confirmed the affiliation of ‘Ca. However evaluating the restriction profiles of 16S rRNA gene in digital RFLP evaluation revealed that the Spilanthes pressure associated was associated to phytoplasma subgroup 16SrI-B.

That is the primary report of 16SrI-B group phytoplasm
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